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recombinant human il6 protein r d systems 206 il  (R&D Systems)


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    R&D Systems recombinant human il6 protein r d systems 206 il
    Recombinant Human Il6 Protein R D Systems 206 Il, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 921 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+il6+protein+r+d+systems+206+il/Recombinant+Human+IL-6+Protein/pmc12578408__jci-135-189570-s083-60-113-117
    Average 96 stars, based on 921 article reviews
    recombinant human il6 protein r d systems 206 il - by Bioz Stars, 2026-09
    96/100 stars

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    Concentration Assay:

    Article Title: Endothelial STING and STAT1 mediate IFN-independent effects of IL-6 in an endotoxemia-induced model of shock
    Article Snippet: .. Reagent or disposable Vendor Catalog Number Concentration/ Dose Tamoxifen Sigma Aldrich T5648 2 mg in 100 μL Peanut Oil Sigma Aldrich P2144 N/A Lipopolysaccharides Sigma Aldrich L4391 250 μg/ 250 μL Collagenase Type 1 Worthington Biochemicals LS004196 0.20% RNAse A Qiagen 19101 100 mg/ml Dispase II Roche 10295825001 0.20% DNeasy Blood and Tissue kit Qiagen 69504 As per instructions RNEasy Plus Micro Kit Qiagen 74034 As per instructions BD Falcon cell strainer 70um pore size Thermo Fisher 352350 N/A Dynabeads M-280 Streptavidin Thermo Scientific 11205D 1mg/100 μL Immunohistochemistry ABC kit Elite Vector Labs PK-6100 N/A ImmPACT DAB Vector Labs SK-4105 N/A VectaMount Vector Labs H-5000 N/A Fetal bovine serum Sigma Aldrich F4135 5% Recombinant Human IL6 protein R&D Systems 206-IL-200/CF 200 ng/mL Recombinant human IL6r alpha R&D Systems 227-SR-025/CF 100 ng/mL Phenol red-free EBM 2 media PromoCell C-22211 N/A Growth medium 2 supplement mix Promocell C-39216 1X Complete Endothelial Cell Medium /w Kit Cell Biologics H1168 1X EndoGRO-MV Complete Culture Media Kit Millipore Sigma SCME004 1X Penicillin-Streptomycin Solution, 100x Corning 30-002-CI 1X Gelatin Millipore Sigma ES-006-B 0.10% 0.05% Trypsin-EDTA Gibco 25300054 1x Ruxolitinib Selleck Chem S1378 2 μM Cycloheximide Sigma 357420010 5 μg/ml DMSO Millipore Sigma 472301 <0.1% RU-521 Cayman Chemicals 31765 10 μM 2'3' cGAMP Cayman Chemicals 19887 30 μg/ml Recombinant Human IFN-alpha 2 R&D Systems 11105-1 2000 units/ml Recombinant Human IFN beta protein R&D Systems 8499-IF-010/CF 500 units/ml TNF R&D Systems 210-TA-020 20 ng/ml Lipopolysaccharides Sigma Aldrich L4391 1 μg/ml Endothelial Cell Growth Supplement Discovery Labware 356006 5 mg/mL Heparin sodium salt from porcine intestinal mucosa Sigma H3393 10 mg/mL Sodium Pyruvate (100mM) Gibco 11360-070 1% GlutaMAX (100X) Gibco A12860-01 1% MEM Non-Essential Amino Acids (100X) Gibco 11140-050 1% HEPES (1M) GIbco 15630-080 2.50% Dynabeads Sheep anti-Rat igG Invitrogen 11035 0.5 mg/mL Complete protease inhibitor mixture Roche Applied 11697498001 1X Mice Supplemental Table 1 - Commercial sources for critical reagents and their catalog numbers .. and Immunoblotting PhosSTOP phosphatase inhibitor mixture Roche Applied 4906845001 1X Sodium fluoride Millipore Sigma S-1504 100 mM Phenyl arsine oxide Millipore Sigma P-3075 100 μM Sodium pyrophosphate decahydrate Millipore Sigma 221368 10 mM Sodium orthovanadate Millipore Sigma S6508 100 μM Transblot Turbo RTA Mini Nitrocellulose - Transfer kit Bio-Rad 1704270 N/A Clarity Western ECL Substrate Bio-Rad 1705061 As per instructions Clarity Max Western ECL Substrate Bio-Rad 1705062 As per instructions Bovine Serum Albumin Rockland Immunochemicals BSA-1000 1-5% 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) Roche 10236276001 1 μg/ml Triton X Sigma T8787 0.1% PrimeScript RT Master Mix Clontech RR036B 1X TRIzol reagent Invitrogen 15596018 1X iTaq Universal SYBR Green Supermix Bio Rad 1725125 1X Rneasy Plus Micro Kit Qiagen 74034 N/A Lipofectamine RNAiMAX transfection reagent Invitrogen 13778150 6 pmol siRNA/μl lipid ON-TARGETplus non-targeting control pool Horizon Discovery D-001810-10-20 50 nM Opti-MEM Thermo Fisher Scientific 31985070 1X ON-TARGETplus Human STAT1 siRNA, smartpool Horizon Discovery Biosciences L-003543-00-0005 50 nM ON-TARGETplus Human STAT3 siRNA, smartpool Horizon Discovery Biosciences L-003544-00-0005 50 nM ON-TARGETplus Human IFNAR1 siRNA, smartpool Horizon Discovery Biosciences L-020209-00-0020 50 nM ON-TARGETplus Human TMEM173 (STING) siRNA, smartpool Horizon Discovery Biosciences L-024333-00-0005 50 nM ON-TARGETplus Human IRF1 siRNA, smartpool Horizon Discovery Biosciences L-011704-00-0020 50 nM ON-TARGETplus Human IRF2 siRNA, smartpool Horizon Discovery Biosciences L-019668-00-0020 50 nM ON-TARGETplus Human IRF3 siRNA, smartpool Horizon Discovery Biosciences L-006875-00-0005 50 nM ON-TARGETplus Human IRF4 siRNA, smartpool Horizon Discovery Biosciences L-011705-02-0020 50 nM ON-TARGETplus Human TMEM173 (CGAS) siRNA, smartpool Horizon Discovery Biosciences L-015607-02-0010 50 nM On Target plus non-targeting control pool Horizon Discovery Biosciences D-001810-10-20 50 nM Avanti Polar Lipids, 1,2-diheptanlyl-snglycerol Avanti Polar Lipids NC9999043 300 mM RNAsin Plus Promega PAN2615 40 U/ml Protein G beads Thermo Fisher/Invitrogen 10004D 375 μl Ab for TRAP Memorial Sloan Kettering CC HTZGFP-19C8 50 μg

    Pore Size:

    Article Title: Endothelial STING and STAT1 mediate IFN-independent effects of IL-6 in an endotoxemia-induced model of shock
    Article Snippet: .. Reagent or disposable Vendor Catalog Number Concentration/ Dose Tamoxifen Sigma Aldrich T5648 2 mg in 100 μL Peanut Oil Sigma Aldrich P2144 N/A Lipopolysaccharides Sigma Aldrich L4391 250 μg/ 250 μL Collagenase Type 1 Worthington Biochemicals LS004196 0.20% RNAse A Qiagen 19101 100 mg/ml Dispase II Roche 10295825001 0.20% DNeasy Blood and Tissue kit Qiagen 69504 As per instructions RNEasy Plus Micro Kit Qiagen 74034 As per instructions BD Falcon cell strainer 70um pore size Thermo Fisher 352350 N/A Dynabeads M-280 Streptavidin Thermo Scientific 11205D 1mg/100 μL Immunohistochemistry ABC kit Elite Vector Labs PK-6100 N/A ImmPACT DAB Vector Labs SK-4105 N/A VectaMount Vector Labs H-5000 N/A Fetal bovine serum Sigma Aldrich F4135 5% Recombinant Human IL6 protein R&D Systems 206-IL-200/CF 200 ng/mL Recombinant human IL6r alpha R&D Systems 227-SR-025/CF 100 ng/mL Phenol red-free EBM 2 media PromoCell C-22211 N/A Growth medium 2 supplement mix Promocell C-39216 1X Complete Endothelial Cell Medium /w Kit Cell Biologics H1168 1X EndoGRO-MV Complete Culture Media Kit Millipore Sigma SCME004 1X Penicillin-Streptomycin Solution, 100x Corning 30-002-CI 1X Gelatin Millipore Sigma ES-006-B 0.10% 0.05% Trypsin-EDTA Gibco 25300054 1x Ruxolitinib Selleck Chem S1378 2 μM Cycloheximide Sigma 357420010 5 μg/ml DMSO Millipore Sigma 472301 <0.1% RU-521 Cayman Chemicals 31765 10 μM 2'3' cGAMP Cayman Chemicals 19887 30 μg/ml Recombinant Human IFN-alpha 2 R&D Systems 11105-1 2000 units/ml Recombinant Human IFN beta protein R&D Systems 8499-IF-010/CF 500 units/ml TNF R&D Systems 210-TA-020 20 ng/ml Lipopolysaccharides Sigma Aldrich L4391 1 μg/ml Endothelial Cell Growth Supplement Discovery Labware 356006 5 mg/mL Heparin sodium salt from porcine intestinal mucosa Sigma H3393 10 mg/mL Sodium Pyruvate (100mM) Gibco 11360-070 1% GlutaMAX (100X) Gibco A12860-01 1% MEM Non-Essential Amino Acids (100X) Gibco 11140-050 1% HEPES (1M) GIbco 15630-080 2.50% Dynabeads Sheep anti-Rat igG Invitrogen 11035 0.5 mg/mL Complete protease inhibitor mixture Roche Applied 11697498001 1X Mice Supplemental Table 1 - Commercial sources for critical reagents and their catalog numbers .. and Immunoblotting PhosSTOP phosphatase inhibitor mixture Roche Applied 4906845001 1X Sodium fluoride Millipore Sigma S-1504 100 mM Phenyl arsine oxide Millipore Sigma P-3075 100 μM Sodium pyrophosphate decahydrate Millipore Sigma 221368 10 mM Sodium orthovanadate Millipore Sigma S6508 100 μM Transblot Turbo RTA Mini Nitrocellulose - Transfer kit Bio-Rad 1704270 N/A Clarity Western ECL Substrate Bio-Rad 1705061 As per instructions Clarity Max Western ECL Substrate Bio-Rad 1705062 As per instructions Bovine Serum Albumin Rockland Immunochemicals BSA-1000 1-5% 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) Roche 10236276001 1 μg/ml Triton X Sigma T8787 0.1% PrimeScript RT Master Mix Clontech RR036B 1X TRIzol reagent Invitrogen 15596018 1X iTaq Universal SYBR Green Supermix Bio Rad 1725125 1X Rneasy Plus Micro Kit Qiagen 74034 N/A Lipofectamine RNAiMAX transfection reagent Invitrogen 13778150 6 pmol siRNA/μl lipid ON-TARGETplus non-targeting control pool Horizon Discovery D-001810-10-20 50 nM Opti-MEM Thermo Fisher Scientific 31985070 1X ON-TARGETplus Human STAT1 siRNA, smartpool Horizon Discovery Biosciences L-003543-00-0005 50 nM ON-TARGETplus Human STAT3 siRNA, smartpool Horizon Discovery Biosciences L-003544-00-0005 50 nM ON-TARGETplus Human IFNAR1 siRNA, smartpool Horizon Discovery Biosciences L-020209-00-0020 50 nM ON-TARGETplus Human TMEM173 (STING) siRNA, smartpool Horizon Discovery Biosciences L-024333-00-0005 50 nM ON-TARGETplus Human IRF1 siRNA, smartpool Horizon Discovery Biosciences L-011704-00-0020 50 nM ON-TARGETplus Human IRF2 siRNA, smartpool Horizon Discovery Biosciences L-019668-00-0020 50 nM ON-TARGETplus Human IRF3 siRNA, smartpool Horizon Discovery Biosciences L-006875-00-0005 50 nM ON-TARGETplus Human IRF4 siRNA, smartpool Horizon Discovery Biosciences L-011705-02-0020 50 nM ON-TARGETplus Human TMEM173 (CGAS) siRNA, smartpool Horizon Discovery Biosciences L-015607-02-0010 50 nM On Target plus non-targeting control pool Horizon Discovery Biosciences D-001810-10-20 50 nM Avanti Polar Lipids, 1,2-diheptanlyl-snglycerol Avanti Polar Lipids NC9999043 300 mM RNAsin Plus Promega PAN2615 40 U/ml Protein G beads Thermo Fisher/Invitrogen 10004D 375 μl Ab for TRAP Memorial Sloan Kettering CC HTZGFP-19C8 50 μg

    Immunohistochemistry:

    Article Title: Endothelial STING and STAT1 mediate IFN-independent effects of IL-6 in an endotoxemia-induced model of shock
    Article Snippet: .. Reagent or disposable Vendor Catalog Number Concentration/ Dose Tamoxifen Sigma Aldrich T5648 2 mg in 100 μL Peanut Oil Sigma Aldrich P2144 N/A Lipopolysaccharides Sigma Aldrich L4391 250 μg/ 250 μL Collagenase Type 1 Worthington Biochemicals LS004196 0.20% RNAse A Qiagen 19101 100 mg/ml Dispase II Roche 10295825001 0.20% DNeasy Blood and Tissue kit Qiagen 69504 As per instructions RNEasy Plus Micro Kit Qiagen 74034 As per instructions BD Falcon cell strainer 70um pore size Thermo Fisher 352350 N/A Dynabeads M-280 Streptavidin Thermo Scientific 11205D 1mg/100 μL Immunohistochemistry ABC kit Elite Vector Labs PK-6100 N/A ImmPACT DAB Vector Labs SK-4105 N/A VectaMount Vector Labs H-5000 N/A Fetal bovine serum Sigma Aldrich F4135 5% Recombinant Human IL6 protein R&D Systems 206-IL-200/CF 200 ng/mL Recombinant human IL6r alpha R&D Systems 227-SR-025/CF 100 ng/mL Phenol red-free EBM 2 media PromoCell C-22211 N/A Growth medium 2 supplement mix Promocell C-39216 1X Complete Endothelial Cell Medium /w Kit Cell Biologics H1168 1X EndoGRO-MV Complete Culture Media Kit Millipore Sigma SCME004 1X Penicillin-Streptomycin Solution, 100x Corning 30-002-CI 1X Gelatin Millipore Sigma ES-006-B 0.10% 0.05% Trypsin-EDTA Gibco 25300054 1x Ruxolitinib Selleck Chem S1378 2 μM Cycloheximide Sigma 357420010 5 μg/ml DMSO Millipore Sigma 472301 <0.1% RU-521 Cayman Chemicals 31765 10 μM 2'3' cGAMP Cayman Chemicals 19887 30 μg/ml Recombinant Human IFN-alpha 2 R&D Systems 11105-1 2000 units/ml Recombinant Human IFN beta protein R&D Systems 8499-IF-010/CF 500 units/ml TNF R&D Systems 210-TA-020 20 ng/ml Lipopolysaccharides Sigma Aldrich L4391 1 μg/ml Endothelial Cell Growth Supplement Discovery Labware 356006 5 mg/mL Heparin sodium salt from porcine intestinal mucosa Sigma H3393 10 mg/mL Sodium Pyruvate (100mM) Gibco 11360-070 1% GlutaMAX (100X) Gibco A12860-01 1% MEM Non-Essential Amino Acids (100X) Gibco 11140-050 1% HEPES (1M) GIbco 15630-080 2.50% Dynabeads Sheep anti-Rat igG Invitrogen 11035 0.5 mg/mL Complete protease inhibitor mixture Roche Applied 11697498001 1X Mice Supplemental Table 1 - Commercial sources for critical reagents and their catalog numbers .. and Immunoblotting PhosSTOP phosphatase inhibitor mixture Roche Applied 4906845001 1X Sodium fluoride Millipore Sigma S-1504 100 mM Phenyl arsine oxide Millipore Sigma P-3075 100 μM Sodium pyrophosphate decahydrate Millipore Sigma 221368 10 mM Sodium orthovanadate Millipore Sigma S6508 100 μM Transblot Turbo RTA Mini Nitrocellulose - Transfer kit Bio-Rad 1704270 N/A Clarity Western ECL Substrate Bio-Rad 1705061 As per instructions Clarity Max Western ECL Substrate Bio-Rad 1705062 As per instructions Bovine Serum Albumin Rockland Immunochemicals BSA-1000 1-5% 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) Roche 10236276001 1 μg/ml Triton X Sigma T8787 0.1% PrimeScript RT Master Mix Clontech RR036B 1X TRIzol reagent Invitrogen 15596018 1X iTaq Universal SYBR Green Supermix Bio Rad 1725125 1X Rneasy Plus Micro Kit Qiagen 74034 N/A Lipofectamine RNAiMAX transfection reagent Invitrogen 13778150 6 pmol siRNA/μl lipid ON-TARGETplus non-targeting control pool Horizon Discovery D-001810-10-20 50 nM Opti-MEM Thermo Fisher Scientific 31985070 1X ON-TARGETplus Human STAT1 siRNA, smartpool Horizon Discovery Biosciences L-003543-00-0005 50 nM ON-TARGETplus Human STAT3 siRNA, smartpool Horizon Discovery Biosciences L-003544-00-0005 50 nM ON-TARGETplus Human IFNAR1 siRNA, smartpool Horizon Discovery Biosciences L-020209-00-0020 50 nM ON-TARGETplus Human TMEM173 (STING) siRNA, smartpool Horizon Discovery Biosciences L-024333-00-0005 50 nM ON-TARGETplus Human IRF1 siRNA, smartpool Horizon Discovery Biosciences L-011704-00-0020 50 nM ON-TARGETplus Human IRF2 siRNA, smartpool Horizon Discovery Biosciences L-019668-00-0020 50 nM ON-TARGETplus Human IRF3 siRNA, smartpool Horizon Discovery Biosciences L-006875-00-0005 50 nM ON-TARGETplus Human IRF4 siRNA, smartpool Horizon Discovery Biosciences L-011705-02-0020 50 nM ON-TARGETplus Human TMEM173 (CGAS) siRNA, smartpool Horizon Discovery Biosciences L-015607-02-0010 50 nM On Target plus non-targeting control pool Horizon Discovery Biosciences D-001810-10-20 50 nM Avanti Polar Lipids, 1,2-diheptanlyl-snglycerol Avanti Polar Lipids NC9999043 300 mM RNAsin Plus Promega PAN2615 40 U/ml Protein G beads Thermo Fisher/Invitrogen 10004D 375 μl Ab for TRAP Memorial Sloan Kettering CC HTZGFP-19C8 50 μg

    Recombinant:

    Article Title: Endothelial STING and STAT1 mediate IFN-independent effects of IL-6 in an endotoxemia-induced model of shock
    Article Snippet: .. Reagent or disposable Vendor Catalog Number Concentration/ Dose Tamoxifen Sigma Aldrich T5648 2 mg in 100 μL Peanut Oil Sigma Aldrich P2144 N/A Lipopolysaccharides Sigma Aldrich L4391 250 μg/ 250 μL Collagenase Type 1 Worthington Biochemicals LS004196 0.20% RNAse A Qiagen 19101 100 mg/ml Dispase II Roche 10295825001 0.20% DNeasy Blood and Tissue kit Qiagen 69504 As per instructions RNEasy Plus Micro Kit Qiagen 74034 As per instructions BD Falcon cell strainer 70um pore size Thermo Fisher 352350 N/A Dynabeads M-280 Streptavidin Thermo Scientific 11205D 1mg/100 μL Immunohistochemistry ABC kit Elite Vector Labs PK-6100 N/A ImmPACT DAB Vector Labs SK-4105 N/A VectaMount Vector Labs H-5000 N/A Fetal bovine serum Sigma Aldrich F4135 5% Recombinant Human IL6 protein R&D Systems 206-IL-200/CF 200 ng/mL Recombinant human IL6r alpha R&D Systems 227-SR-025/CF 100 ng/mL Phenol red-free EBM 2 media PromoCell C-22211 N/A Growth medium 2 supplement mix Promocell C-39216 1X Complete Endothelial Cell Medium /w Kit Cell Biologics H1168 1X EndoGRO-MV Complete Culture Media Kit Millipore Sigma SCME004 1X Penicillin-Streptomycin Solution, 100x Corning 30-002-CI 1X Gelatin Millipore Sigma ES-006-B 0.10% 0.05% Trypsin-EDTA Gibco 25300054 1x Ruxolitinib Selleck Chem S1378 2 μM Cycloheximide Sigma 357420010 5 μg/ml DMSO Millipore Sigma 472301 <0.1% RU-521 Cayman Chemicals 31765 10 μM 2'3' cGAMP Cayman Chemicals 19887 30 μg/ml Recombinant Human IFN-alpha 2 R&D Systems 11105-1 2000 units/ml Recombinant Human IFN beta protein R&D Systems 8499-IF-010/CF 500 units/ml TNF R&D Systems 210-TA-020 20 ng/ml Lipopolysaccharides Sigma Aldrich L4391 1 μg/ml Endothelial Cell Growth Supplement Discovery Labware 356006 5 mg/mL Heparin sodium salt from porcine intestinal mucosa Sigma H3393 10 mg/mL Sodium Pyruvate (100mM) Gibco 11360-070 1% GlutaMAX (100X) Gibco A12860-01 1% MEM Non-Essential Amino Acids (100X) Gibco 11140-050 1% HEPES (1M) GIbco 15630-080 2.50% Dynabeads Sheep anti-Rat igG Invitrogen 11035 0.5 mg/mL Complete protease inhibitor mixture Roche Applied 11697498001 1X Mice Supplemental Table 1 - Commercial sources for critical reagents and their catalog numbers .. and Immunoblotting PhosSTOP phosphatase inhibitor mixture Roche Applied 4906845001 1X Sodium fluoride Millipore Sigma S-1504 100 mM Phenyl arsine oxide Millipore Sigma P-3075 100 μM Sodium pyrophosphate decahydrate Millipore Sigma 221368 10 mM Sodium orthovanadate Millipore Sigma S6508 100 μM Transblot Turbo RTA Mini Nitrocellulose - Transfer kit Bio-Rad 1704270 N/A Clarity Western ECL Substrate Bio-Rad 1705061 As per instructions Clarity Max Western ECL Substrate Bio-Rad 1705062 As per instructions Bovine Serum Albumin Rockland Immunochemicals BSA-1000 1-5% 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) Roche 10236276001 1 μg/ml Triton X Sigma T8787 0.1% PrimeScript RT Master Mix Clontech RR036B 1X TRIzol reagent Invitrogen 15596018 1X iTaq Universal SYBR Green Supermix Bio Rad 1725125 1X Rneasy Plus Micro Kit Qiagen 74034 N/A Lipofectamine RNAiMAX transfection reagent Invitrogen 13778150 6 pmol siRNA/μl lipid ON-TARGETplus non-targeting control pool Horizon Discovery D-001810-10-20 50 nM Opti-MEM Thermo Fisher Scientific 31985070 1X ON-TARGETplus Human STAT1 siRNA, smartpool Horizon Discovery Biosciences L-003543-00-0005 50 nM ON-TARGETplus Human STAT3 siRNA, smartpool Horizon Discovery Biosciences L-003544-00-0005 50 nM ON-TARGETplus Human IFNAR1 siRNA, smartpool Horizon Discovery Biosciences L-020209-00-0020 50 nM ON-TARGETplus Human TMEM173 (STING) siRNA, smartpool Horizon Discovery Biosciences L-024333-00-0005 50 nM ON-TARGETplus Human IRF1 siRNA, smartpool Horizon Discovery Biosciences L-011704-00-0020 50 nM ON-TARGETplus Human IRF2 siRNA, smartpool Horizon Discovery Biosciences L-019668-00-0020 50 nM ON-TARGETplus Human IRF3 siRNA, smartpool Horizon Discovery Biosciences L-006875-00-0005 50 nM ON-TARGETplus Human IRF4 siRNA, smartpool Horizon Discovery Biosciences L-011705-02-0020 50 nM ON-TARGETplus Human TMEM173 (CGAS) siRNA, smartpool Horizon Discovery Biosciences L-015607-02-0010 50 nM On Target plus non-targeting control pool Horizon Discovery Biosciences D-001810-10-20 50 nM Avanti Polar Lipids, 1,2-diheptanlyl-snglycerol Avanti Polar Lipids NC9999043 300 mM RNAsin Plus Promega PAN2615 40 U/ml Protein G beads Thermo Fisher/Invitrogen 10004D 375 μl Ab for TRAP Memorial Sloan Kettering CC HTZGFP-19C8 50 μg

    Protease Inhibitor:

    Article Title: Endothelial STING and STAT1 mediate IFN-independent effects of IL-6 in an endotoxemia-induced model of shock
    Article Snippet: .. Reagent or disposable Vendor Catalog Number Concentration/ Dose Tamoxifen Sigma Aldrich T5648 2 mg in 100 μL Peanut Oil Sigma Aldrich P2144 N/A Lipopolysaccharides Sigma Aldrich L4391 250 μg/ 250 μL Collagenase Type 1 Worthington Biochemicals LS004196 0.20% RNAse A Qiagen 19101 100 mg/ml Dispase II Roche 10295825001 0.20% DNeasy Blood and Tissue kit Qiagen 69504 As per instructions RNEasy Plus Micro Kit Qiagen 74034 As per instructions BD Falcon cell strainer 70um pore size Thermo Fisher 352350 N/A Dynabeads M-280 Streptavidin Thermo Scientific 11205D 1mg/100 μL Immunohistochemistry ABC kit Elite Vector Labs PK-6100 N/A ImmPACT DAB Vector Labs SK-4105 N/A VectaMount Vector Labs H-5000 N/A Fetal bovine serum Sigma Aldrich F4135 5% Recombinant Human IL6 protein R&D Systems 206-IL-200/CF 200 ng/mL Recombinant human IL6r alpha R&D Systems 227-SR-025/CF 100 ng/mL Phenol red-free EBM 2 media PromoCell C-22211 N/A Growth medium 2 supplement mix Promocell C-39216 1X Complete Endothelial Cell Medium /w Kit Cell Biologics H1168 1X EndoGRO-MV Complete Culture Media Kit Millipore Sigma SCME004 1X Penicillin-Streptomycin Solution, 100x Corning 30-002-CI 1X Gelatin Millipore Sigma ES-006-B 0.10% 0.05% Trypsin-EDTA Gibco 25300054 1x Ruxolitinib Selleck Chem S1378 2 μM Cycloheximide Sigma 357420010 5 μg/ml DMSO Millipore Sigma 472301 <0.1% RU-521 Cayman Chemicals 31765 10 μM 2'3' cGAMP Cayman Chemicals 19887 30 μg/ml Recombinant Human IFN-alpha 2 R&D Systems 11105-1 2000 units/ml Recombinant Human IFN beta protein R&D Systems 8499-IF-010/CF 500 units/ml TNF R&D Systems 210-TA-020 20 ng/ml Lipopolysaccharides Sigma Aldrich L4391 1 μg/ml Endothelial Cell Growth Supplement Discovery Labware 356006 5 mg/mL Heparin sodium salt from porcine intestinal mucosa Sigma H3393 10 mg/mL Sodium Pyruvate (100mM) Gibco 11360-070 1% GlutaMAX (100X) Gibco A12860-01 1% MEM Non-Essential Amino Acids (100X) Gibco 11140-050 1% HEPES (1M) GIbco 15630-080 2.50% Dynabeads Sheep anti-Rat igG Invitrogen 11035 0.5 mg/mL Complete protease inhibitor mixture Roche Applied 11697498001 1X Mice Supplemental Table 1 - Commercial sources for critical reagents and their catalog numbers .. and Immunoblotting PhosSTOP phosphatase inhibitor mixture Roche Applied 4906845001 1X Sodium fluoride Millipore Sigma S-1504 100 mM Phenyl arsine oxide Millipore Sigma P-3075 100 μM Sodium pyrophosphate decahydrate Millipore Sigma 221368 10 mM Sodium orthovanadate Millipore Sigma S6508 100 μM Transblot Turbo RTA Mini Nitrocellulose - Transfer kit Bio-Rad 1704270 N/A Clarity Western ECL Substrate Bio-Rad 1705061 As per instructions Clarity Max Western ECL Substrate Bio-Rad 1705062 As per instructions Bovine Serum Albumin Rockland Immunochemicals BSA-1000 1-5% 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) Roche 10236276001 1 μg/ml Triton X Sigma T8787 0.1% PrimeScript RT Master Mix Clontech RR036B 1X TRIzol reagent Invitrogen 15596018 1X iTaq Universal SYBR Green Supermix Bio Rad 1725125 1X Rneasy Plus Micro Kit Qiagen 74034 N/A Lipofectamine RNAiMAX transfection reagent Invitrogen 13778150 6 pmol siRNA/μl lipid ON-TARGETplus non-targeting control pool Horizon Discovery D-001810-10-20 50 nM Opti-MEM Thermo Fisher Scientific 31985070 1X ON-TARGETplus Human STAT1 siRNA, smartpool Horizon Discovery Biosciences L-003543-00-0005 50 nM ON-TARGETplus Human STAT3 siRNA, smartpool Horizon Discovery Biosciences L-003544-00-0005 50 nM ON-TARGETplus Human IFNAR1 siRNA, smartpool Horizon Discovery Biosciences L-020209-00-0020 50 nM ON-TARGETplus Human TMEM173 (STING) siRNA, smartpool Horizon Discovery Biosciences L-024333-00-0005 50 nM ON-TARGETplus Human IRF1 siRNA, smartpool Horizon Discovery Biosciences L-011704-00-0020 50 nM ON-TARGETplus Human IRF2 siRNA, smartpool Horizon Discovery Biosciences L-019668-00-0020 50 nM ON-TARGETplus Human IRF3 siRNA, smartpool Horizon Discovery Biosciences L-006875-00-0005 50 nM ON-TARGETplus Human IRF4 siRNA, smartpool Horizon Discovery Biosciences L-011705-02-0020 50 nM ON-TARGETplus Human TMEM173 (CGAS) siRNA, smartpool Horizon Discovery Biosciences L-015607-02-0010 50 nM On Target plus non-targeting control pool Horizon Discovery Biosciences D-001810-10-20 50 nM Avanti Polar Lipids, 1,2-diheptanlyl-snglycerol Avanti Polar Lipids NC9999043 300 mM RNAsin Plus Promega PAN2615 40 U/ml Protein G beads Thermo Fisher/Invitrogen 10004D 375 μl Ab for TRAP Memorial Sloan Kettering CC HTZGFP-19C8 50 μg

    Mouse Assay:

    Article Title: Endothelial STING and STAT1 mediate IFN-independent effects of IL-6 in an endotoxemia-induced model of shock
    Article Snippet: .. Reagent or disposable Vendor Catalog Number Concentration/ Dose Tamoxifen Sigma Aldrich T5648 2 mg in 100 μL Peanut Oil Sigma Aldrich P2144 N/A Lipopolysaccharides Sigma Aldrich L4391 250 μg/ 250 μL Collagenase Type 1 Worthington Biochemicals LS004196 0.20% RNAse A Qiagen 19101 100 mg/ml Dispase II Roche 10295825001 0.20% DNeasy Blood and Tissue kit Qiagen 69504 As per instructions RNEasy Plus Micro Kit Qiagen 74034 As per instructions BD Falcon cell strainer 70um pore size Thermo Fisher 352350 N/A Dynabeads M-280 Streptavidin Thermo Scientific 11205D 1mg/100 μL Immunohistochemistry ABC kit Elite Vector Labs PK-6100 N/A ImmPACT DAB Vector Labs SK-4105 N/A VectaMount Vector Labs H-5000 N/A Fetal bovine serum Sigma Aldrich F4135 5% Recombinant Human IL6 protein R&D Systems 206-IL-200/CF 200 ng/mL Recombinant human IL6r alpha R&D Systems 227-SR-025/CF 100 ng/mL Phenol red-free EBM 2 media PromoCell C-22211 N/A Growth medium 2 supplement mix Promocell C-39216 1X Complete Endothelial Cell Medium /w Kit Cell Biologics H1168 1X EndoGRO-MV Complete Culture Media Kit Millipore Sigma SCME004 1X Penicillin-Streptomycin Solution, 100x Corning 30-002-CI 1X Gelatin Millipore Sigma ES-006-B 0.10% 0.05% Trypsin-EDTA Gibco 25300054 1x Ruxolitinib Selleck Chem S1378 2 μM Cycloheximide Sigma 357420010 5 μg/ml DMSO Millipore Sigma 472301 <0.1% RU-521 Cayman Chemicals 31765 10 μM 2'3' cGAMP Cayman Chemicals 19887 30 μg/ml Recombinant Human IFN-alpha 2 R&D Systems 11105-1 2000 units/ml Recombinant Human IFN beta protein R&D Systems 8499-IF-010/CF 500 units/ml TNF R&D Systems 210-TA-020 20 ng/ml Lipopolysaccharides Sigma Aldrich L4391 1 μg/ml Endothelial Cell Growth Supplement Discovery Labware 356006 5 mg/mL Heparin sodium salt from porcine intestinal mucosa Sigma H3393 10 mg/mL Sodium Pyruvate (100mM) Gibco 11360-070 1% GlutaMAX (100X) Gibco A12860-01 1% MEM Non-Essential Amino Acids (100X) Gibco 11140-050 1% HEPES (1M) GIbco 15630-080 2.50% Dynabeads Sheep anti-Rat igG Invitrogen 11035 0.5 mg/mL Complete protease inhibitor mixture Roche Applied 11697498001 1X Mice Supplemental Table 1 - Commercial sources for critical reagents and their catalog numbers .. and Immunoblotting PhosSTOP phosphatase inhibitor mixture Roche Applied 4906845001 1X Sodium fluoride Millipore Sigma S-1504 100 mM Phenyl arsine oxide Millipore Sigma P-3075 100 μM Sodium pyrophosphate decahydrate Millipore Sigma 221368 10 mM Sodium orthovanadate Millipore Sigma S6508 100 μM Transblot Turbo RTA Mini Nitrocellulose - Transfer kit Bio-Rad 1704270 N/A Clarity Western ECL Substrate Bio-Rad 1705061 As per instructions Clarity Max Western ECL Substrate Bio-Rad 1705062 As per instructions Bovine Serum Albumin Rockland Immunochemicals BSA-1000 1-5% 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) Roche 10236276001 1 μg/ml Triton X Sigma T8787 0.1% PrimeScript RT Master Mix Clontech RR036B 1X TRIzol reagent Invitrogen 15596018 1X iTaq Universal SYBR Green Supermix Bio Rad 1725125 1X Rneasy Plus Micro Kit Qiagen 74034 N/A Lipofectamine RNAiMAX transfection reagent Invitrogen 13778150 6 pmol siRNA/μl lipid ON-TARGETplus non-targeting control pool Horizon Discovery D-001810-10-20 50 nM Opti-MEM Thermo Fisher Scientific 31985070 1X ON-TARGETplus Human STAT1 siRNA, smartpool Horizon Discovery Biosciences L-003543-00-0005 50 nM ON-TARGETplus Human STAT3 siRNA, smartpool Horizon Discovery Biosciences L-003544-00-0005 50 nM ON-TARGETplus Human IFNAR1 siRNA, smartpool Horizon Discovery Biosciences L-020209-00-0020 50 nM ON-TARGETplus Human TMEM173 (STING) siRNA, smartpool Horizon Discovery Biosciences L-024333-00-0005 50 nM ON-TARGETplus Human IRF1 siRNA, smartpool Horizon Discovery Biosciences L-011704-00-0020 50 nM ON-TARGETplus Human IRF2 siRNA, smartpool Horizon Discovery Biosciences L-019668-00-0020 50 nM ON-TARGETplus Human IRF3 siRNA, smartpool Horizon Discovery Biosciences L-006875-00-0005 50 nM ON-TARGETplus Human IRF4 siRNA, smartpool Horizon Discovery Biosciences L-011705-02-0020 50 nM ON-TARGETplus Human TMEM173 (CGAS) siRNA, smartpool Horizon Discovery Biosciences L-015607-02-0010 50 nM On Target plus non-targeting control pool Horizon Discovery Biosciences D-001810-10-20 50 nM Avanti Polar Lipids, 1,2-diheptanlyl-snglycerol Avanti Polar Lipids NC9999043 300 mM RNAsin Plus Promega PAN2615 40 U/ml Protein G beads Thermo Fisher/Invitrogen 10004D 375 μl Ab for TRAP Memorial Sloan Kettering CC HTZGFP-19C8 50 μg



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    Potency and Selectivity of LW402 Assayed in WBAs

    Journal: Journal of Inflammation Research

    Article Title: Preclinical Characterization of the Selective JAK1 Inhibitor LW402 for Treatment of Rheumatoid Arthritis

    doi: 10.2147/JIR.S301076

    Figure Lengend Snippet: Potency and Selectivity of LW402 Assayed in WBAs

    Article Snippet: Blood from healthy volunteers who had given informed consent was collected into sodium heparin tubes after preincubation with compounds at 37°C for 30 minutes, triggered with recombinant human IL6 (100 ng/mL; R&D Systems), human GM-CSF (20 ng/mL; PeproTech), or vehicle (PBS plus 0.1% BSA) for 20 minutes at 37°C, then treated with lysis/fix buffer (BD) for 10 minutes to lyse red blood cells and fix leukocytes.

    Techniques: